{{cart_num}}
My CartApplication：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：3.9 pg/ml
Storage：2-8℃，6 months
Gene ID：16159
Qty：
Size：
| Name | Mouse IL-12 p70 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-12 p70 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 7.81-500 pg/ml |
| Sensitivity | 3.9 pg/ml |
| Specificity | Do not react with Mouse IL-1α，2，3，4，6，7，9，10 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P43431 |
| Gene ID | 16159 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗小鼠IL-12 p70单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的小鼠IL-12 p70会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗小鼠IL-12 p70抗体和辣根过氧化物酶（HRP）标记的链霉亲和素，两者结合物会与板子上包被抗体捕获的标准品和样本中的小鼠IL-12 p70发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的小鼠IL-12 p70，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的小鼠IL-12 p70浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中小鼠IL-12 p70的浓度。 |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Reversing Immune Evasion using a DNA Nano-orchestrator for Pancreatic Cancer Immunotherapy
Click to check >>Author：Xiaotian Zhao, Yuanmin Dong, Jing Zhang, Chen Chen, Lin Gao, Chongdeng Shi, Zhipeng Fu, Maosen Han, Chunwei Tang, Peng Sun, Zhenmei Yang, Cai Zhang, Kun Zhao, Xinyi Jiang
IF：10.6330
Publish_to：Acta Biomaterialia
PMID：37150276
Lentinan-laden microspheres reprogram the tumor microenvironment and improve anti-PD-L1 efficacy
Click to check >>Author：Haixin Wang, Xiao-Dong Gao, Hua Yue
IF：7.5710
Publish_to：Journal of Materials Chemistry B
PMID：37159051
Cascade targeting codelivery of ingenol-3-angelate and doxorubicin for enhancing cancer chemoimmunotherapy through synergistic effects in prostate cancer
Click to check >>Author：王志成
IF：7.3480
Publish_to：Materials Today Bio
PMID：34977528
