Yeast Lytic Enzyme
Cat.No：R1020 Solarbio
Storage：Store at -20℃，1 year.
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> Molecular Biology > Molecular Cloning-Related Enzymes > Nucleic Acid Extraction Related Enzymes > Yeast Lytic Enzyme 
Product Information
| Name | Yeast Lytic Enzyme |
| Storage | Store at -20℃，1 year. |
| Unit | Bottle |
| Specification | 1.25ml |
Yeast wall breaking enzyme solution
Item number: R1020
Product content:
Ingredient name specification saved
Yeast wall breaking enzyme solution 1.25 mL-20℃
Sorbitol buffer 25mL RT
β-mercaptoethanol 0.3mL RT
Save:
Enzyme stored at -20℃, sorbitol buffer at room temperature, valid for one year.
Product Description:
Yeast wall breaking enzyme is a mixture of snail enzyme, cellulase and other enzymes, which is mainly used for the treatment of yeast cell wall.
This product is 50% glycerin solution. Generally, 1-5 ml yeast culture (no more than 5×107 cells) is collected after centrifugation, adding 25μL yeast wall breaking enzyme, 470μL sorbitol buffer, and then adding 5μL β-mercaptoethanol, and it can be treated at 30℃for 1-2 hours (if it is combined with glass bead crushing method, the effect will be better, especially for extracting nucleic acids with very little content such as plasmids).
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Direct Visualization and Restoration of Metallic Ion-Induced Subcellular Ultrastructural Remodeling
Click to check >>Author：Zheng Dang, Xia-Yu Tao, Yong Guan, Zhao Wu, Ying Xiong, Gang Liu, YangChao Tian, Li-Jiao Tian
IF：18.0270
Publish_to：ACS Nano
PMID：37156644
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