Korthof Medium Base
Cat.No:LA7850 Solarbio
Storage:RT
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Name | Korthof Medium Base |
Storage | RT |
Grade | BR |
Unit | Bottle |
Specification | 250g |
Product introduction
For the growth culture of leptospira.
Preparation method
Weigh 3.38g of this product, autoclave it in 1000ml of distilled water at 121℃ for 20 minutes, cool it to 50-55℃, add 8ml of sterile rabbit (horse or sheep) serum, mix it well, hold it in a water bath at 56℃ for 30 minutes, after passing the sterility test, set aside. To prevent contamination, cefsuloidime sodium can be selectively added 0.5mg/ml.
Composition: g/L
Peptone: 0.8
Sodium chloride: 1.4
Sodium bicarbonate: 0.02
Potassium chloride: 0.04
Potassium dihydrogen phosphate: 0.24
Disodium hydrogen phosphate: 0.88
Hydrochloric acid: 1mg
Vitamin B12:1mg
pH7.2±0.1, 25℃
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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