Apigenin

Examples of using this product（for reference only）

HPLC

Apigenin, scutellarein, scutellarin, and apigenin-7-O-glucuronide were measured at 335 nm using a Kinetex 5 μm Biphenyl 100 ? LC Column (250 × 4.6 mm; Phenomenex, USA) operating at 30 ℃. The mobile phase consisted of 0.1% formic acid and acetonitrile with methanol at a flow rate of 1 ml min?1 using the following gradients: 0–20 min, 22% acetonitrile, 5% methanol; 20–22 min, acetonitrile increased from 22 to 90%; 25 min, 22% acetonitrile. Subsequently, the column was washed and equilibrated for 5 min before the next injection. Thirty microliters of the sample were injected into the HPLC system and each run was stopped at 30 min after the injection.

References：

Liu X, Cheng J, Zhang G, Ding W, Duan L, Yang J, Kui L, Cheng X, Ruan J, Fan W, Chen J, Long G, Zhao Y, Cai J, Wang W, Ma Y, Dong Y, Yang S, Jiang H. Engineering yeast for the production of breviscapine by genomic analysis and synthetic biology approaches. Nat Commun. 2018 Jan 31;9(1):448. doi: 10.1038/s41467-018-02883-z. PMID: 29386648; PMCID: PMC5792594.

UPLC-MS

Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) was performed with liquid mass spectrometer for evaluation. The determination was performed on the SB-C18 column at the flow rate of 0.3 mL/min and the column temperature was 40 ℃. The flavonoid and anthocyanin components were detected at 210 nm.

References：

Shi M, Ali MM, He Y, Ma S, Rizwan HM, Yang Q, Li B, Lin Z, Chen F. Flavonoids Accumulation in Fruit Peel and Expression Profiling of Related Genes in Purple (Passiflora edulis f. edulis) and Yellow (Passiflora edulis f. flavicarpa) Passion Fruits. Plants (Basel). 2021 Oct 20;10(11):2240. doi: 10.3390/plants10112240. PMID: 34834602; PMCID: PMC8620868.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.