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My CartReactivity：Rat,Mouse,Human
Application：IF,IHC,WB,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：23468
Host：Mouse
Qty：
Size：
| Name | Anti-CBX5 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG2b |
| Clone Number | 3G2-H10-A6 |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. IF 1:50-200. ICC 1:50-200. |
| Target | CBX5 |
| Background | This gene encodes a highly conserved nonhistone protein, which is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. The protein has a single N-terminal chromodomain which can bind to histone proteins via methylated lysine residues, and a C-terminal chromo shadow-domain (CSD) which is responsible for the homodimerization and interaction with a number of chromatin-associated nonhistone proteins. The encoded product is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. The gene has a pseudogene located on chromosome 3. Multiple alternatively spliced variants, encoding the same protein, have been identified. |
| Swiss Prot | P45973 |
| Gene ID | 23468 |
| Subcellular Location | Chromosome Nucleus centromere |
| Immunogen | Recombinant protein of human CBX5 |
| Calculated MW | 22kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Rat Mouse Human |
| Application | IF IHC ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with CBX5 antibody diluted at 1:1000;Lane: MCF-7 293T 3T3 C6 COS7 and mouse brain.
Immunohistochemistry of paraffin-embedded Human breast carcinoma with CBX5 antibody diluted at 1:100.
Immunofluorescence analysis of HeLa using CBX5 antibody diluted at 1:100.
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