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My CartReactivity：Human
Application：IF,FCM,WB,ICC
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze / thaw cycles.
Gene ID：4090
Host：Mouse
Qty：
Size：
| Name | Anti-SMAD5 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 4B10-B10-B6 |
| Dilution Ratio | WB 1:500-1000. IF 1:50-200. FCM 1:50-100. ICC 1:50-200. |
| Target | SMAD5 |
| Background | SMAD5 is a member of the Mothers Against Dpp (MAD)-related family of proteins. It is a receptor-regulated SMAD (R-SMAD), and acts as an intracellular signal transducer for the transforming growth factor beta superfamily. SMAD5 is activated through serine phosphorylation by BMP (bone morphogenetic proteins) type 1 receptor kinase. It is cytoplasmic in the absence of its ligand and migrates into the nucleus upon phosphorylation and complex formation with SMAD4. Here the SMAD5/SMAD4 complex stimulates the transcription of target genes. |
| Swiss Prot | Q99717 |
| Gene ID | 4090 |
| Subcellular Location | Cytoplasm Nucleus |
| Immunogen | Recombinant protein of human SMAD5 |
| Calculated MW | 52kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，0.5% BSA，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human |
| Application | IF FCM ICC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with SMAD5 antibody diluted at 1:1000;Lane: Jurkat K562 HeLa.
Immunofluorescence analysis of HeLa using SMAD5 antibody diluted at 1:100.
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