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My CartReactivity：Human,Mouse
Application：WB,IHC
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：6093
Host：Rabbit
Qty：
Size：
| Name | Anti-ROCK1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-1000. IHC 1:50-100. |
| Target | ROCK1 |
| Background | This gene encodes a protein serine/threonine kinase that is activated when bound to the GTP-bound form of Rho. The small GTPase Rho regulates formation of focal adhesions and stress fibers of fibroblasts, as well as adhesion and aggregation of platelets and lymphocytes by shuttling between the inactive GDP-bound form and the active GTP-bound form. Rho is also essential in cytokinesis and plays a role in transcriptional activation by serum response factor. This protein, a downstream effector of Rho, phosphorylates and activates LIM kinase, which in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. A pseudogene, related to this gene, is also located on chromosome 18. |
| Swiss Prot | Q13464 |
| Gene ID | 6093 |
| Subcellular Location | Cell membrane Cell projection Cytoplasm Golgi apparatus membrane Peripheral membrane protein bleb centriole centrosome cytoskeleton lamellipodium microtubule organizing center ruffle |
| Immunogen | Recombinant protein of human ROCK1 |
| Public Immunogen Range | 400-660aa |
| Calculated MW | 158kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Mouse |
| Application | IHC WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded colorectal cancer��hu�� using ROCK1 antibody at dilution of 1:100 (x100 lens��x200 lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug ROCK1 antibody.
Western blot analysis with ROCK1 antibody diluted at 1:1000;Lane: HeLa COS-7.
Immunohistochemistry of paraffin-embedded mouse spleen with ROCK1 antibody diluted at 1:75.
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