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My CartReactivity：Mouse,Rat,Human
Application：IF,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：5033
Host：Rabbit
Qty：
Size：
| Name | Anti-P4HA1 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:100-500. IF 1:50-200. |
| Target | P4HA1 |
| Background | This gene encodes a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains. Alternatively spliced transcript variants encoding different isoforms have been described. |
| Swiss Prot | P13674 |
| Gene ID | 5033 |
| Subcellular Location | Endoplasmic reticulum lumen |
| Immunogen | A synthetic peptide of human P4HA1 |
| Public Immunogen Range | 235-534aa |
| Calculated MW | 61kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Rat Human |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Ovarian cancer(hu) using P4HA1 antibody at dilution of 1:100 (100x lens).
Western blot analysis with P4HA1 antibody diluted at 1:1500;Lane: HepG2 NCI-H460 U-251MG.
Immunofluorescence analysis of L929 cells using P4HA1 antibody diluted at 1:100.
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