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My CartReactivity：Mouse,Human
Application：IF,WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：5530
Host：Rabbit
Qty：
Size：
| Name | Anti-PPP3CA Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IF 1:50-200. |
| Target | PPP3CA |
| Background | In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases. In general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunit have been identified, designated PP1, PP2A, PP2B and PP2C. An additional protein phosphatase catalytic subunit, PPX (also known as PP4), is a putative member of a novel PP family. The PP2B family comprises subfamily members PP2B-A, PP2B-A∫ and PP2B-A. Two additional regulatory subunits been identified, designated PP2B-B1 and PP2B-B2. |
| Swiss Prot | Q08209 |
| Gene ID | 5530 |
| Subcellular Location | Cell membrane Cell projection Cytoplasm Membrane |
| Immunogen | Recombinant protein of human PPP3CA |
| Public Immunogen Range | 1-511aa |
| Calculated MW | 59kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Colorectal cancer(hu) using PPP3CA antibody at dilution of 1:100 (x100 lens��x200 lens).
Western blot analysis with PPP3CA antibody diluted at 1:1000;Lane: HeLa SH-SY5Y THP-1 A549 Mouse brain Mouse lung.
Immunofluorescence analysis of HeLa cells using PPP3CA antibody diluted at 1:100.
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