Soil Neutral Protease Activity Assay Kit

Determination of Significance：

Soil protease is involved in the transformation of amino acids, proteins and other organic compounds containing protein nitrogen in soil. Those hydrolytic product is one of the nitrogen sources of higher plants. Soil neutral protease catalyzes the hydrolysis of protein in neutral environment, which is related to soil organic matter content, nitrogen and other soil properties. Under neutral conditions, soil neutral protease could hydrolyze casein to produce tyrosine. In alkaline conditions, tyrosine reduced phosphomolybdic acid compound to form tungsten blue with absorbance peak in 680 nm.

Measurement Principle：

Soil protease is involved in the transformation of amino acids, proteins and other organic compounds containing protein nitrogen in soil. Those hydrolytic product is one of the nitrogen sources of higher plants. Soil neutral protease catalyzes the hydrolysis of protein in neutral environment, which is related to soil organic matter content, nitrogen and other soil properties. Under neutral conditions, soil neutral protease could hydrolyze casein to produce tyrosine. In alkaline conditions, tyrosine reduced phosphomolybdic acid compound to form tungsten blue with absorbance peak in 680 nm.

Self Provided：

Spectrophotometer, water bath, transferpettor, 1 mL glass cuvette, methylbenzene, distilled water, 50 mesh sieve(or smaller).

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.