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Storage:Store at -20℃，6 months
Determination of Significance：
Mitochondrial Respiratory Chain Complex Ⅰ (EC 1.6.5.3) same as NADH-CoQ reductase or NADH dehydrogenase, exists widely in mitochondria of animals, plants, microorganisms and cultured cells, which is the largest protein complex in inner mitochondrial membrane. This enzyme catalyzes a pair of electrons transfer from NADH to CoQ, reduce O2 to O2-, which is the main site of O2- production on the respiratory electron transport chain. The enzyme activity of Complex Ⅰ reflects the state of respiratory electron transport chain (ETC) and produce state of ROS.
Measurement Principle：
Complex Ⅰ catalyzes NADH to form NAD+, the activity of this enzyme can be calculated by detecting the oxidation rate of NADH at 340 nm.
Self Provided：
Ultraviolet spectrophotometer, water bath/constant temperature incubator, desk centrifuge, adjustable pipette, 1 mL quartz cuvette, mortar/homogenizer/cell ultrasonic crusher, acetone, ice and distilled water.
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