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My CartReactivity：Mouse,Human
Application：WB
Clone Type：Polyclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：2048
Host：Rabbit
Qty：
Size：
| Name | Anti-EPHB2 Polyclonal Antibody |
| Host | Rabbit |
| Clone Type | Polyclonal Antibody |
| Subtype | IgG |
| Dilution Ratio | WB 1:500-2000. IHC 1:50-200. |
| Target | EPHB2 |
| Background | This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors are composed of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. They bind ligands called ephrins and are involved in diverse cellular processes including motility, division, and differentiation. A distinguishing characteristic of Eph-ephrin signaling is that both receptors and ligands are competent to transduce a signaling cascade, resulting in bidirectional signaling. This protein belongs to a subgroup of the Eph receptors called EphB. Proteins of this subgroup are distinguished from other members of the family by sequence homology and preferential binding affinity for membrane-bound ephrin-B ligands. Allelic variants are associated with prostate and brain cancer susceptibility. Alternative splicing results in multiple transcript variants. |
| Swiss Prot | P29323 |
| Gene ID | 2048 |
| Subcellular Location | Cell membrane Cell projection Single-pass type I membrane protein axon dendrite |
| Immunogen | Recombinant protein of human EPHB2 |
| Public Immunogen Range | 250-540aa |
| Calculated MW | 117kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide，50% glycerol，pH7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Y-Dilution Ratio | "WB 1:500-2000." |
| Specification | 50ul 100ul |
| Reactivity | Mouse Human |
| Application | WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Immunohistochemistry of paraffin-embedded Colorectal cancer(hu) using EPHB2 antibody at dilution of 1:100 (x100 lens,x200 lens).
Western blot analysis with EPHB2 antibody diluted at 1:1000;Lane: NCI-H460 HepG2 HeLa HT-1080 SW620 Mouse brain Mouse heart.
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