NAD-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit
Cat.No:BC1040 Solarbio
Detection Method:Spectrophotometer
Storage:Store at -20℃,6 months
Qty:
Size:
{{cart_num}}
My Cart>
Biochemical Assay Kits >
Protein & Enzyme Series >
Coenzyme I Series >
NAD-Malate Dehydrogenase(NAD-MDH) Activity Assay KitDetection Method:Spectrophotometer
Storage:Store at -20℃,6 months
Qty:
Size:
Name | NAD-Malate Dehydrogenase(NAD-MDH) Activity Assay Kit |
Detection Method | Spectrophotometer |
Storage | Store at -20℃,6 months |
Unit | Kit |
Specification | 50T/48S |
Determination of Significance:
Malate Dehydrogenase (MDH, EC 1.1.1.37)is widely exist in animal, plant, microbe and cells culture. MDH in mitochondria is one of the key enzymes of TCA cycle, which catalyzes the formation of oxaloacetic acid from malic acid. In contrast, MDH in serum catalyzes the formation of malic acid from oxaloacetic acid. Oxaloacetic acid is an important intermediate that connects several important metabolic pathways. MDH plays an important role in many physiological activities of cells, including mitochondrial energy metabolism, malic acid-aspartic acid shuttle system, reactive oxygen species metabolism and disease resistance. According to the different coenzyme specificity, MDH is divided into NAD- dependent MDH and NADP- dependent MDH. Bacteria usually only contain NAD-MDH. NAD-MDH is distributed in the cytoplasm and mitochondria in eukaryotic cells.
Measurement Principle:
NAD-MDH catalyzes NADH to reduce oxaloacetic acid into malic acid, which resulting in a decrease in absorption at 340nm.
Self Provided:
Ultraviolet spectrophotometer,desk centrifuge, water-bath, adjustablepipette, 1mL quartzcuvette, distilled water.
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Sorry, there is no experimental images.
Sorry, there is no more information.
Hemocytes in blue mussel Mytilus edulis adopt different energy supply modes to cope with different BDE-47 exposures
Click to check >>Author:Yongshun Jiang, Sai Cao, Bin Zhou, Qiyue Cao, Mengxue Xu, Tianli Sun, Xinyu Zhao, Zhongyuan Zhou, You Wang
IF:10.7530
Publish_to:SCIENCE OF THE TOTAL ENVIRONMENT
PMID:37146804
Exogenously applied zinc improves sugar-acid profile of loquat (Eriobotrya japonica Lindl.) by regulating enzymatic activities and expression of their metabolism-related genes
Click to check >>Author:Muhammad Moaaz Ali, Shaista Gull, Xiaobo Hu, Youming Hou, Faxing Chen
IF:6.5000
Publish_to:PLANT PHYSIOLOGY AND BIOCHEMISTRY
PMID:37329690
AoMae1 Regulates Hyphal Fusion,Lipid Droplet Accumulation,Conidiation,and Trap Formation in Arthrobotrys oligospora
Click to check >>Author:Yankun Liu, Meichen Zhu, Wenjie Wang, Xuemei Li, Na Bai, Meihua Xie, Jinkui Yang
IF:5.7240
Publish_to:Journal of Fungi
PMID:37108952
Unraveling the Antibacterial Mechanism of Plasma-Activated Lactic Acid against Pseudomonas ludensis by Untargeted Metabolomics
Click to check >>Author:Zhaobin Wang, Xiaoting Wang, Xiaowei Sheng, Luling Zhao, Jing Qian, Jianhao Zhang, Jin Wang
IF:5.5610
Publish_to:Foods
PMID:37107401