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Xanthine Oxidase(XOD) Assay Kit

BC1090-241212Manual

Cat.No：BC1090 Solarbio

Detection Method：Spectrophotometer;Spectrophotometer

Storage：Store at 2-8℃，6 months

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Product Information

Detection Method	Spectrophotometer;Spectrophotometer
Storage	Store at 2-8℃，6 months
Unit	Kit
Specification	50T/48S

Determination of Significance：

XOD (EC 1.17.3.2) catalyzes the oxidation of hypoxanthine to xanthine and superoxide anion, which is one of the main sources of active oxygen and is also one of the key enzymes of nucleotide metabolism. XOD is mainly distributed in mammalian heart, lung, liver and other tissues. When liver function impaired, XOD is released into serum in a large amount, which has specific significance for the diagnosis of liver damage.

Measurement Principle：

XOD catalyzes hypoxanthine to produce xanthine and superoxide anion. Superoxide anion reacts with hydroxylamine hydrochloride to form NO^2-, and the NO^2- under the action of p-aminobenzenesulfonamide and naphthalene ethylenediamine hydrochloride is produced a red azo compound with a characteristic absorption peak at 530 nm. The color depth is linear with XOD activity.

Self Provided：

Spectrophotometer, constant temperature foster box/water-bath, centrifuge, 1mL glass cuvette, adjustable transferpettor, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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