Determination of Significance:
Glutathione is a natural tripeptide composed of glutamic acid (Glu), cysteine (Cys) and glycine (Gly). It is a kind of compound containing sulfhydryl group (-SH), which widely exists in animal tissue, plant tissue, microorganism and yeast.
Measurement Principle:
Glutathione can react with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB) to produce 2-nitro-5-mercaptobenzoic acid and glutathione disulfide (GSSG). 2-nitro-5-mercaptobenzoic acid is a yellow product with the maximum absorption at 412 nm.
Self Provided:
Analytical balance, mortar/homogenizer, low temperature centrifuge, water bath, adjustable pipette, spectrophotometer, 1 mL glass cuvette and distilled water.
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