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Determination of Significance：
γ-glutamyltranspeptidase (γ-GT) is a key enzyme in γ-glutanyl cycle, which catalyzes the degradation of GSH. γ-GT catalyzes the transfer of γ-glutamyl groups from GSH or other γ-glutamyl compounds to receptors. It can also catalyze the hydrolysis of GSH andother γ-glutamyl compounds to produce glutamate, which plays an important role in the metabolism of extracellular glutathione.
Measurement Principle：
γ-GT catalyzes the transfer of γ-glutamyl in glutamyl p-nitroaniline to N-glycylglycine to form p-nitroaniline with characteristic light absorption at 405 nm.γ-GT enzyme activity is calculated by measuring the increase rate of light absorption at 405nm.
Self Provided：
Spectrophotometer, centrifuge, water-bath,adjustable pipette, 1mL glass cuvette, ice, mortar/homogenizer and distilled water.
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