Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit
Cat.No：BC1550 Solarbio
Detection Method：Spectrophotometer
Storage：Store at 2-8℃，6 months
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> Biochemical Assay Kits > Nitrogen Metabolism Series > Amino Acid Metabolism Series > Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit
Product Information
| Name | Glutamic-pyruvic Transaminase(GPT) Activity Assay Kit |
| Detection Method | Spectrophotometer |
| Storage | Store at 2-8℃，6 months |
| Unit | Kit |
| Specification | 50T/24S |
Determination of Significance：
GPT is widely found in animals, plants, microbes and cultured cells, which is an important enzyme in amino acid metabolism. It catalyzes the transamination of amino acid and keto acid. In addition, GPT activity is very high in Mammalian liver cells. GPT is released into the blood when liver cells necrotic, serum GPT activity is significantly increased. Therefore, GPT is recommended as the most sensitive indicator of liver damage by the World Health Organization.
Measurement Principle：
GPT catalyzes the transamination reaction of alanine and α-ketoglutarate to generate pyruvate and glutamic acid; the addition of 2,4-dinitrophenylhydrazine solution not only terminates the above reaction, but also increases Into phenylpyrene pyruvate; which shows brownish red in alkaline condition, the activity of GOT enzyme activity can be calculated by measuring the absorbance of 505 nm.
Self Provided：
Spectrophotometer, water bath, 1 mL glass cuvette, desk centrifuge, transferpettor, distilled water, ice and mortar/homogenizer.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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