Determination of Significance:
Animal liver and kidney are the main organs of amino acid metabolism, so the changes of amino acids in urine can reflect the physiological state of liver and kidney. In addition, amino acids can also respond to burns, typhoid and other aspects of the situation. The content of amino acids in plants have a great significance to the study of nitrogen’s metabolism, absorption, transport, assimilation and nutritional status under different conditions and at different stages of growth and development.
Measurement Principle:
α-Amino of amino acid can react with hydrated ninhydrin to produce blue purple compound, which has absorption peak at 570 nm, and the content of amino acid is calculated by measuring absorbance of 570 nm.
Self Provided:
Desk centrifuge, spectrophotometer, water bath, 1 mL glass cuvette, transferpettor, mortar/homogenizer, absolute ethyl alcohol, ice and distilled water.
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这个酶标仪可以测吗2022-08-18 12:01:51
Administrator您好,酶标仪检测有BC1575微量法体系的试剂盒,如果用分光光度法这个盒子最后想用酶标仪检测不要调整缩减反应体系,最后混匀取200微升到酶标板中用酶标仪去读数。