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Storage:Store at -20℃，6 months
Determination of Significance：
Mitochondrial complex III (EC 1.10.2.2), also known as CoQ-cytochrome C reductase, is widely found in mitochondria of animals, plants, microorganisms and cultured cells. It is a common component of the main pathway and branch of the mitochondrial respiratory electron transport chain. Mitochondrial complex III is responsible for transferring hydrogen from reduced CoQ to cytochrome C and then produce reduced cytochrome C.
Measurement Principle：
Unlike oxidized cytochrome C, reduced cytochrome C has a characteristic absorption at 550 nm, so the rate of increase in light absorption at 550 nm can reflect mitochondrial complex III enzyme activity.
Self Provided：
Spectrophotometer, water bath, desk centrifuge, transferpettor, mortar/homogenizer/cell ultrasonic crusher, 1ml glass cuvette, ice and distilled water.
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