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My Cart> Antibodies > Loading Control Antibodies > Mitochondria > Anti-COX4I1 Monoclonal AntibodyReactivity：Human,Rat,Mouse
Application：IF,WB
Clone Type：Monoclonal Antibody
Storage：Store at -20℃. Avoid freeze/thaw cycles.
Gene ID：1327
Host：Mouse
Qty：
Size：
| Name | Anti-COX4I1 Monoclonal Antibody |
| Host | Mouse |
| Clone Type | Monoclonal Antibody |
| Subtype | IgG1 |
| Clone Number | 6C8 |
| Dilution Ratio | WB 1:1000-3000. IF 1:100-300. |
| Target | COX4I1 |
| Background | The enzyme cytochrome c oxidase or Complex IV, EC 1.9.3.1) is a large transmembrane protein complex found in bacteria and the mitochondrion.It is the last enzyme in the respiratory electron transport chain of mitochondria (or bacteria) located in the mitochondrial (or bacterial) membrane. It receives an electron from each of four cytochrome c molecules, and transfers them to one oxygen molecule, converting molecular oxygen to two molecules of water. In the process, it binds four protons from the inner aqueous phase to make water, and in addition translocates four protons across the membrane, helping to establish a transmembrane difference of proton electrochemical potential that the ATP synthase then uses to synthesize ATP. |
| Swiss Prot | P13073 |
| Gene ID | 1327 |
| Subcellular Location | Cytoplasm Mitochondrion inner membrane |
| Immunogen | recombinant protein |
| Calculated MW | 20kDa |
| Purification Method | Affinity purification |
| Storage Buffer | PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage | Store at -20℃. Avoid freeze/thaw cycles. |
| Unit | Piece |
| Specification | 50ul 100ul |
| Reactivity | Human Rat Mouse |
| Application | IF WB |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Kindly reminder：Quick spin before use. If you have any questions, please contact us.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
Western blot analysis with COX4I1 antibody diluted at 1:3000;Lane: HepG2,Mouse heart,Mouse skeletal muscle,Rat heart.
Immunofluorescence analysis of Hela cells using COX4I1 antibody diluted at 1:200.
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