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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：7.8 pg/ml
Storage：2-8℃，6 months
Gene ID：25296
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| Name | Rat BMP4 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | BMP4 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.62-1000 pg/ml |
| Sensitivity | 7.8 pg/ml |
| Specificity | Do not react with Rat BMP4 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | Q06826 |
| Gene ID | 25296 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠BMP4单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠BMP4会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠BMP4抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠BMP4发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠BMP4，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠BMP4浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠BMP4的浓度。 |
| Background | BMP-4 is a TGF-beta superfamily ligand that is widely expressed from early embryogenesis through adulthood. It plays an important role in mesenchyme formation，epidermal determination，suppression of neural induction，the development of multiple organs，and tissue repair. The mouse BMP-4 precursor contains a 273?amino acid (aa) propeptide and a 116?aa mature protein. The propeptide is cleaved intracellularly by furin or proprotein convertase 6，enabling the 15 kDa mature BMP-4 monomer to form an active disulfide linked homodimer or heterodimer with BMP-7. Mature mouse and human BMP-4 share 98% aa sequence identity. Mouse BMP-4 shares 85%?aa sequence identity with mouse BMP-2 and 35%-54% with other mouse BMPs. Compared to BMP-4 homodimers，BMP-4/BMP-7 heterodimers exhibit a greater potency in inducing osteogenic differentiation. In Xenopus，the heterodimers can also induce the formation of mesoderm，whereas BMP-4 homodimers only provide ventralizing signals for existing mesoderm. BMP-4 signals through tetrameric complexes composed of type I (primarily Activin RIA or BMPR-IA) and type II (primarily Activin RIIA or BMPR-II) receptors. The bioavailability of BMP-4 is regulated by its interaction with multiple proteins and glycosaminoglycans. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
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2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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