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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：31.25 pg/ml
Storage：2-8℃，6 months
Gene ID：24179
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| Name | Rat Angiotensinogen ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Angiotensinogen |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 62.5-4000 pg/ml |
| Sensitivity | 31.25 pg/ml |
| Specificity | Do not react with Rat Adiponectin，ApoA1，BMP1，CCL2，CRP，HGF，IL-1β，IL-2，IFNy，MMP-2，，PDGF，TGFβ1，TLR1，TLR9，TNF-α，VEGF and VEGFR1 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | P01015 |
| Gene ID | 24179 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠AGT单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠AGT会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠AGT抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠AGT发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠AGT，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠AGT浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠AGT的浓度。 |
| Background | Serpin A8 (serine?proteinase?inhibitor-clade?A #8; also angiotensinogen/ANGT) is a secreted，52-62 kDa glycoprotein member of the clade F-subfamily，serpin superfamily of protease inhibitors.? It is expressed by neurons and hepatocytes，and undergoes extracellular cleavage by renin to create a ten amino acid (aa) peptide termed Ang/angiotensin I. ?This inactive peptide is further cleaved by ACE on the endothelial cell membrane to create bioactive Ang II.? Ang II induces vascoconstriction and aldosterone release.? Mature human Serpin A8 is 452 aa in length (aa 34-485).? It contains Ang I (aa 34-43) that is cleaved to create Ang II (aa 34-41).? Serpin A8 may circulate in a 200 kDa complex with major basic protein (MBP)，or as part of a larger 300 kDa complex with MBP and complement C3dg. ?Over aa 34-485，human Serpin A8 shares 61% aa identity with mouse Serpin A8. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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