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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：3pg/mL
Storage：2-8℃，6 months
Qty：
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| Name | Rat PGE2 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | PGE2 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15-1，000pg/mL |
| Sensitivity | 3pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠PGE2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠PGE2会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠PGE2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠PGE2发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠PGE2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠PGE2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠PGE2的浓度。 |
| Background | Prostaglandin E2 (PGE2) is formed in a variety of cells from prostaglandin H2，which is synthesized from arachidonic acid by the enzyme prostaglandin synthetase. PGE2 has a number of biological actions，including vasodilation，both anti- and proinflammatory action，modulation of sleep/wake cycles，and facilitation of human immunodeficiency virus replication. It elevates cAMP levels，stimulates bone resorption and has thermoregulatory effects. It has been shown to be a regulator of sodium excretion and renal hemodynamics. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Pharmacological activation of the Nrf2 pathway by Taxifolin remodels articular cartilage microenvironment for the therapy of Osteoarthritis
Click to check >>Author：Hongyi Jiang, Jiachen Yu, Zijian Yan, Zhongnan Lin, Minghao Lin, Yiwen Mao, Zechao Hong, Jian Lin, Xinghe Xue, Xiaoyun Pan
IF：5.6000
Publish_to：INTERNATIONAL IMMUNOPHARMACOLOGY
PMID：37399606
