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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：31.25 pg/ml
Storage：2-8℃，6 months
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| Name | Rat IL-12 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-12 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 62.5-4000 pg/ml |
| Sensitivity | 31.25 pg/ml |
| Specificity | Do not react with Rat IL-12 analogue etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠IL-12单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠IL-12会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠IL-12抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠IL-12发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠IL-12，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠IL-12浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠IL-12的浓度。 |
| Background | Interleukin 12 (IL-12) is the founding member of the IL-12 family of heterodimeric cytokines，which have important immunological functions. IL-12 is composed of two?disulfide-linked subunits of 35?kDa and 40?kDa，respectively. The 35?kDa subunit (p35) is an alpha -helical protein homologous to IL-6 and G?CSF. The 40 kDa subunit (p40) contains one fibronectin type III and one Ig C2-like domain，and has a high degree of structural homology to type I cytokine receptors. Whereas p35 subunit is unique to IL-12，the p40 subunit is also utilized in IL-23. Mature rat p35 is a 194 amino acids (aa) protein that is secreted as a heterodimer linked to p40. It contains three potential N-linked glycosylation sites and shares 86%，and 58% aa sequence identity with mouse and human p35，respectively. Mature rat p40 contains 313 aa and can exist in multiple forms，including monomer，homodimer，heterodimer linked to p19 (forming IL-23)，and heterodimer linked to p35 (forming IL-12). Mature rat p40 shows 92% and 66% aa sequence identity to mouse and human p40，respectively. Cells known to produce IL-12 include macrophages，dendritic cells，monocytes，Langerhans cells，neutrophils，and keratinocytes. The activities of IL-12 are mediated by the receptor complex composed of two?type?I transmembrane proteins: a 100?kDa ligand-binding subunit (IL?12?R beta 1) and a 130?kDa signal transducing subunit (IL?12?R beta 2).?IL?12 facilitates hematopoietic stem cell proliferation，induces NK cell proliferation，and potentiates the expansion and late activation of Th1 CD4+?T cells. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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