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My CartApplication：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：39 pg/ml
Storage：2-8℃，6 months
Gene ID：397660
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| Name | Porcine Adiponectin ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Adiponectin |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 78.12-5000 pg/ml |
| Sensitivity | 39 pg/ml |
| Specificity | Do not react with ApoAI，BMP1，BMP2，BMP4，BMP5，BMP7，CCL2，CCL4，CCL5，CRP，HGF，HSP27，IL-1β，IL1R1，IL-2，IL-4，IL-5，IL-6，IL-8，IL-10，IL-12，IL-13，IL-15，IL-17C，IL-21，IL-23，IFN-β，IGF1，MMP-2，MMP-9，PDGF，serpinE1，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TLR9，TNF-α，TNFRI，TNFRII，sIL2R，sIL6R，VEGF，VEGFR1 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Gene ID | 397660 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪adiponectin单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的猪adiponectin会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗猪adiponectin抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的猪adiponectin发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的猪adiponectin，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的猪adiponectin浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中猪adiponectin的浓度。 |
| Background | Adipose cells produce and secrete numerous physiologically important proteins，such as lipoprotein lipase (LPL)，leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation，mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production，thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha，it is currently being investigated as an important regulator of whole body energy homeostasis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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