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Application：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：25pg/mL
Storage：2-8℃，6 months
Gene ID：397320
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| Name | Porcine MMP-1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | MMP-1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 125-8000pg/mL |
| Sensitivity | 25pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P21692 |
| Gene ID | 397320 |
| Background | Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase)，can degrade a broad range of substrates including types I，II，III，VII，VIII，and X collagens as well as casein，gelatin，alpha ?1 antitrypsin，myelin basic protein，L-Selectin，pro-TNF，IL-1 beta，IGF-BP3，IGF-BP5，pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling，characterized by the cleavage of the interstitial collagen triple helix into ?，? fragments. However，as the list of substrates above illustrates，the role of MMP-1 is more diverse than originally envisaged，and may involve enzyme cascades，cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts，keratinocytes，endothelial cells，monocytes and macrophages. Structurally，MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.' |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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