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My CartApplication：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：93 pg/ml
Storage：2-8℃，6 months
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| Name | Porcine MMP-2 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | MMP-2 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 187.5-12000 pg/ml |
| Sensitivity | 93 pg/ml |
| Specificity | Do not react with BMP1，BMP2，BMP4，HGF，IL-1β，IL-2，IL-4，IL-5，IL-6，IL-8，IL-10，IL-12，IL-13，IL-15，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TNF-α，VEGF etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪MMP-2单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的猪MMP-2会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗猪MMP-2抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的猪MMP-2发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的猪MMP-2，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的猪MMP-2浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中猪MMP-2的浓度。 |
| Background | The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines，chemokines，growth factors and binding proteins，cell/cell adhesion molecules，and other proteinases. With a few exceptions，MMPs share common structural motifs including a pro-peptide domain，a catalytic domain，a hinge region，and a hemopexin-like domain. Synthesized as pro-enzymes，most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription，pro-enzyme activation，or by their endogenous inhibitors，tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP). |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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