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Application：ELISA
Reactivity：Canine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：5pg/mL
Storage：2-8℃，6 months
Gene ID：403616
Qty：
Size：
| Name | Canine Leptin ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | Leptin |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Canine |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 25-1600pg/mL |
| Sensitivity | 5pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | O02720 |
| Gene ID | 403616 |
| Background | Adipose cells produce and secrete numerous physiologically important proteins，such as lipoprotein lipase (LPL)，leptin and adiponectin protein of 30 kDa. Adiponectin is a circulating protein that is secreted exclusively by differentiated adipocytes. During adipocyte differentiation，mRNA levels of Adiponectin have been shown to be induced over 100-fold. Studies indicate that Adiponectin enhances the ability of sub-physiological levels of insulin to suppress glucose production，thus linking adipose tissue to whole body glucose regulation. Adiponectin function appears to be regulated by phosphatidylinositol-3-kinase (PI3-K) since Adiponectin secretion is blocked by pharmacologic inhibitors of this kinase. mRNA levels of Adiponectin have been shown to be significantly reduced in adipose tissue of obese patients with Type 2 diabetes relative to control subjects. Structural similarities to TNFalpha suggests that Adiponectin may play a role in pathogenesis of insulin resistance in Type 2 diabetes. Because Adiponectin is down-regulated in various forms of obesity and its structural similarity to TNFalpha，it is currently being investigated as an important regulator of whole body energy homeostasis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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