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My CartApplication：ELISA
Reactivity：Sheep
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：46 pg/ml
Storage：2-8℃，6 months
Gene ID：443342
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| Name | Sheep IL-10 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-10 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Sheep |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 93.75-6000 pg/ml |
| Sensitivity | 46 pg/ml |
| Specificity | Do not react with BMP1，BMP2，BMP3，BMP4，IL-2，IL-4，IL-5，IL-6，IL-8，IFN-γ，TGF-β1，TGF-β2，TGF-β3，TLR1，TLR2，TLR3，TNF-α etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Swiss Prot | Q29408 |
| Gene ID | 443342 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗羊IL-10单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的羊IL-10会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗羊IL-10抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的羊IL-10发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的羊IL-10，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的羊IL-10浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中羊IL-10的浓度。 |
| Background | FGF basic/FGF2 is a growth factor that functions in angiogenesis，wound healing，tissue repair，learning and memory，and the morphogenesis of heart，bone，and brain. It is upregulated in response to inflammatory stimuli and in many tumors. FGF basic binds to FGF R1c and 2c. Its bioactivity is modulated by a number of other binding partners including heparin，Integrin alpha V beta 3，soluble FGF R1，FGF-BP，free gangliosides，Thrombospondin，Pentraxin 3/TSG-14，Fibrinogen，alpha 2-Macroglobulin，PDGF，and CXCL4/PF4. These molecules act as cellular coreceptors or adhesion partners，extracellular matrix decoys or reservoirs，and soluble scavengers or chaperones. In particular，the interaction of FGF basic with cell surface heparan sulfate proteoglycans (HSPG) is required for the binding and activation of FGF receptors. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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