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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：39 pg/ml
Storage：2-8℃，6 months
Gene ID：24446
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| Name | Rat HGF ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | HGF |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 78.12-5000 pg/ml |
| Sensitivity | 39 pg/ml |
| Specificity | Do not react with HGFActivat， Human 的HGF，HGFActivator，HGFR/FcChimera，MSP，MSPβchain etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 1.3h |
| Gene ID | 24446 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠HGF单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠HGF会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠HGF抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠HGF发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠HGF，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠HGF浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠HGF的浓度。 |
| Background | HGF (Hepatocyte Growth Factor，Scatter Factor) induces the proliferation and migration of epithelial cells，hepatocytes，chondrocytes，keratinocytes，melanocytes，endothelial cells，and many tumor cells. During organogenesis and tissue repair，HGF promotes epithelial/endothelial morphogenesis by inducing cell scattering and branching tubulogenesis. It also supports insulin production by pancreatic beta cells，neuronal survival，and immune tolerance. HGF is secreted as a propeptide that is activated by uPA or HGF Activator at sites of tissue damage. Its signaling through the receptor HGF R/c-MET is enhanced by its prior binding to heparan sulfate proteoglycans. The serum levels of HGF are elevated in a wide range of pathologies including liver damage，acute kidney failure，myocardial infarction，type 1 diabetes，obesity，and cancer，as well as in the synovial fluid of rheumatoid arthritis patients. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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