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My CartApplication：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：36 pg/ml
Storage：2-8℃，6 months
Gene ID：396842
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| Name | Porcine CRP ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CRP |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 78.12-5000 pg/ml |
| Sensitivity | 36 pg/ml |
| Specificity | Do not react with ApoAI，BMP1，BMP2，BMP3，BMP4，BMP5，BMP7，CRP，FGFacidic，HGF，HSP27，IL-1α，IL-1β，IL-1RA，IL-1RI，IL-2，IL-5，IL-6，IL-8，IL-10，IL-12，IL-13，IL-15，IL-17C，IL-21，IGF1，MMP-2，MMP9，PDGF，PLA2G7，serpinE1，sIL-2R，sIL-6R，TGFβ1，TGFβ2，TGFβ3，TLR1，TLR2，TLR3，TNF-α，TNFRI，TNFRII，VEGF etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Swiss Prot | O19062 |
| Gene ID | 396842 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗猪CRP单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的猪CRP会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗猪CRP抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的猪CRP发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的猪CRP，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的猪CRP浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中猪CRP的浓度。 |
| Background | C-Reactive Protein (CRP)，also known as Pentraxin 1，is a secreted pentameric protein that functions as a sensor and activator for the innate immune response. In humans，it is a major acute-phase protein; its circulating concentration is dramatically elevated at the onset of inflammation. In mice，however，serum CRP levels increase only slightly during inflammation，and the analogous acute phase role is filled by Pentraxin 2. CRP binds，opsonizes，and induces the phagocytosis of bacteria and apoptotic cells. It regulates activation of the classical complement pathway by binding several proteins in the complement cascade as well as Fc gamma RI，Fc gamma RIIA，and Fc gamma RIIB on macrophages and dendritic cells. It also promotes dendritic cell maturation and humoral immunity. In cardiovascular disease，CRP binds to oxidized LDL，exacerbates tissue damage in myocardial infarction，and inhibits the repair of injured vascular endothelium. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
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