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Application：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：13pg/mL
Storage：2-8℃，6 months
Qty：
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| Name | Porcine TLR9 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | TLR9 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 125-8000pg/mL |
| Sensitivity | 13pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | Q5I2M3 |
| Background | TLR9 (Toll receptor 9; also CD289) is a 145-150 kDa member of the Toll-like receptor family of molecules. It is expressed by colonic epithelium，CD123+?plasmacytoid dendritic cells，and transitional B cells，and responds to unmethylated DNA CpG motifs that exhibit either a GTCGTT sequence (in human)，or a GACGTT sequence (in mouse). TLR9 is found in the ER，and translocates to either the cell membrane，or to lysosomes where it binds bacterial DNA. Precursor human TLR9 is a type I transmembrane protein 1032 amino acids (aa) in length. It possesses a 793 aa extracellular region that contains 26 LRRs (aa 26-818)，plus a 193 aa cytoplasmic domain. The full-length 150 kDa form is suggested to be ligand-binding but nonsignaling. The active form is believed to be an 80 kDa cleavage product found in the endosome compartment. There are multiple splice forms. One contains a deletion of aa 2-16，a second possesses an alternate start site at Met58，while a third and fourth show alternative start sites aa 23 and 24 upstream of the standard site. Over aa 64-189，human TLR9 shares 76% aa identity with mouse TLR9. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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