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Application：ELISA
Reactivity：Rabbit
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：5 pg/ml
Storage：2-8℃，6 months
Gene ID：100009017
Qty：
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| Name | Rabbit IL-15 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-15 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rabbit |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 12.5-800 pg/ml |
| Sensitivity | 5 pg/ml |
| Specificity | Do not react with IL-1β，IL-2，IL-4，IL-5，IL-6，IL-8，IL-10，TLR1，TLR2，TNF-α etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 2.33h |
| Swiss Prot | Q3Y5G8 |
| Gene ID | 100009017 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗兔IL-15单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的兔IL-15会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗兔IL-15抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的兔IL-15发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的兔IL-15，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的兔IL-15浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中兔IL-15的浓度。 |
| Background | IL-15 (Interleukin 15) is a cytokine that induces or enhances the differentiation，maintenance，or activation of multiple T cell subsets including NK，NKT，Th17，Treg，and CD8+ memory cells. It also induces dendritic cell differentiation and inflammatory activation，exhibits anti-tumor activity，and inhibits the deposition of lipid in adipocytes. Complexes of IL-15 with cell surface IL-15 R alpha interact with complexes of IL-2 R beta and the Common gamma Chain on adjacent cells. This transpresentation mechanism enables cells to respond to IL-15 even if they do not express IL-15 R alpha. Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells. The activity of circulating IL-15 is limited by its association with soluble IL-15 R alpha. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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