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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：93.75 pg/ml
Storage：2-8℃，6 months
Gene ID：300339
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| Name | Rat MMP-1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | MMP-1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 187.5-12000 pg/ml |
| Sensitivity | 93.75 pg/ml |
| Specificity | Do not react with BMP1，BMP2BMP3，BMP4，IL-1β，IL-4，IL-5，IL-6，IL-8IFN-γ，TGF-β1，TGF-β2，TGF-β3，TLR1，TLR2，TLR3 etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3.5h |
| Swiss Prot | P81563 |
| Gene ID | 300339 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠MMP-1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的大鼠MMP-1会与酶标板上的包被抗体充分结合；洗板后加入生物素化抗大鼠MMP-1抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠MMP-1发生特异性结合；洗板后加入辣根过氧化物酶（HRP）标记的链霉亲和素，生物素与链霉亲和素会发生高强度的非共价结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠MMP-1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠MMP-1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠MMP-1的浓度。 |
| Background | Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-1 (interstitial collagenase)，can degrade a broad range of substrates including types I，II，III，VII，VIII，and X collagens as well as casein，gelatin，alpha ?1 antitrypsin，myelin basic protein，L-Selectin，pro-TNF，IL-1 beta，IGF-BP3，IGF-BP5，pro MMP-2 and pro MMP-9. A significant role of MMP-1 is the degradation of fibrillar collagens in extracellular matrix remodeling，characterized by the cleavage of the interstitial collagen triple helix into ?，? fragments. However，as the list of substrates above illustrates，the role of MMP-1 is more diverse than originally envisaged，and may involve enzyme cascades，cytokine regulation and cell surface molecule modulation. MMP-1 is expressed by fibroblasts，keratinocytes，endothelial cells，monocytes and macrophages. Structurally，MMP-1 may be divided into several distinct domains; a pro-domain which is cleaved upon activation; a catalytic domain containing the zinc binding site; a short hinge region and a carboxyl terminal (hemopexin-like) domain.' |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
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2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
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