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Aconitase（ACO）Activity Assay Kit

BC4485-241212Manual

Cat.No：BC4485 Solarbio

Detection Method：Spectrophotometer/Microplate Reader

Storage：Store at -20℃，6 months

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Product Information

Name	Aconitase（ACO）Activity Assay Kit
Detection Method	Spectrophotometer/Microplate Reader
Storage	Store at -20℃，6 months
Recommend	If 96-well plate is used for determination, 96-well UV plate should be used, recommended item numberYA0602
Unit	Kit
Specification	100T/96S

Determination of Significance：

Aconitase (ACO) is an important intracellular ferritin, which is mainly present in the cytoplasm and mitochondria. ACO catalyzes the reversible reaction of intracellular citric acid to isocitric acid via cis aconitic acid, which plays an important role in maintaining the success of the tricarboxylic acid cycle and the glyoxylic acid cycle.

Measurement Principle：

Aconitase can catalyze isocitrate to produce aconitic acid. Cisaconitic acid has a characteristic absorption peak at 240 nm. The enzyme activity is calculated by measuring the rate of cisaconitic acid production.

Self Provided：

Spectrophotometer/microplate reader, desk centrifuge, water bath/constant temperature incubator, adjustable transferpettor, ultrasonic cell pulverizer, micro quartz cuvette/96 well (UV) plate, mortar/homogenizer, ice and distilled water.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.

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