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My CartApplication：ELISA
Reactivity：Rat
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：10pg/mL
Storage：2-8℃，6 months
Gene ID：25459
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| Name | Rat HMGB1 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | HMGB1 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Rat |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 62.5-4，000pg/mL |
| Sensitivity | 10pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P63159 |
| Gene ID | 25459 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠HMGB1单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品、预稀释的样本、样本稀释液和抗大鼠HMGB1检测抗体，标准品和样本中的大鼠HMGB1与检测抗体的结合物会与酶标板上的包被抗体充分结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的大鼠HMGB1，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的大鼠HMGB1浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠HMGB1的浓度。 |
| Background | HMGB1 is expressed at high levels in almost all cells (2，4). It was originally discovered as a nuclear protein that could bend DNA. Such bending stabilizes nucleosome formation and regulates the expression of select genes upon recruitment by DNA binding proteins (1，?7，?8). It is now known that HMGB1 can also act extracellularly，both as an inflammatory mediator that promotes monocyte migration and cytokine secretion，and as a mediator of T cell-dendritic cell interaction (1，?4，?7，?9，?10). The cytokine activity of HBMG1 is restricted to the HMG B box，(3) while the A box is associated with the helix-loop-helix domain of transcription factors (11). HMBG1 is released in response to cell death and as a secretion product. Although HMBG-1 does not possess a classic signal sequence，it appears to be secreted as an acetylated form via secretory endolysosome exocytosis (6，12). Once secreted，HMGB1 transduces cellular signals through its high affinity receptor，RAGE and，possibly，TLR2 and TLR4 (1，?3，?4). Human HMGB1 is 100% aa identical to canine HMGB1 and 99% aa identical to mouse，rat，bovine and porcine HMGB1，respectively. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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