DH5α-T1 Competent Cell
Cat.No：C1180 Solarbio
Storage：Store at -70℃
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Oscillating Mixing & Stirring & Dispersing Instrument
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> Molecular Biology > Recombinant Clone Expression > Competent Cell > DH5α-T1 Competent Cell
Product Information
| Name | DH5α-T1 Competent Cell |
| Storage | Store at -70℃ |
| Unit | Bag |
| Specification | 10*100ul |
Dh5α-T1 was an improved strain of conventional DH5α. Deletion of nuclease (endA1) gene improves plasmid yield and quality; Recombinase defect type (recA1) reduces the homologous recombination probability of inserted fragments and ensures the stability of inserted DNA. Due to the presence of lacZΔM15 and the deletion of lacIq gene, it is not necessary to add IPTG, but only need to add X-gal to perform the blue-white spot screening experiment based on the principle of α-complementarity. The mutation of DH5α-T1 in the tonA region of the genome enables the strain to acquire resistance to T1 and T5 phage infection. DH5α-T1 receptor cells were made by a special process, and the conversion efficiency of pUC19 plasmid was as high as 108cfu/μg DNA.
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Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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