TBE Buffer,10× (powder)
Cat.No：T1053 Solarbio
Storage：Store at RT，2 years
Appearance：Powder
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> Molecular Biology > Nucleic Acid Electrophoresis > Nucleic Acid Electrophoresis Reagent > TBE Buffer,10× (powder)
Product Information
| Name | TBE Buffer，10× (powder) |
| Appearance | Powder |
| Storage | Store at RT，2 years |
| Unit | Bottle |
| Specification | 1L |
TBE buffer is a buffer salt solution commonly used in biology for nucleic acid electrophoresis, mainly for agarose gel electrophoresis of DNA. The main components of TBE are TRIS-Borate and EDTA, which has strong buffer capacity, is suitable for longer electrophoresis time, high resolution, and good separation effect when electrophoresis fragments less than 1kb. The boric acid content of TBE buffer can affect the efficiency of DNA recovery and the subsequent enzyme reaction, so TAE buffer is recommended for DNA fragment recovery experiments by agar-gel electrophoresis.
This product is TBE buffer premix powder, can be quickly prepared into 10×TBE buffer. The composition of 1×TBE was: 89mM Tris-boric,2 mM EDTA; pH=8.2-8.4.
Usage:
1. Each bag of powder can be prepared into 1000ml of 10×TBE buffer.
2. Deionized water or double steaming water is used as solvent when preparing.
3, take out all the powder in the bag dissolved in 800ml deionized water or distilled water, fixed volume to 1000ml, that is, 10×TBE buffer.
4, the working concentration of TBE buffer is generally 0.5×, and the 10×TBE buffer can be diluted 20 times before electrophoresis.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Related Paper
Detection of Periodontal Pathogens Based on an Integrated Continuous Flow PCR and Capillary Electrophoresis Microfluidic Chip
Click to check >>Author：Bo Yang, Jiaxin Huang, Chunxian Tao, Zhenqing Li, Dawei Zhang, Yoshinori Yamaguchi
IF：3.3440
Publish_to：Separations
PMID：
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