Western Transfer Buffer,10× (powder)

Electrophoretic transfer buffers are used as buffers for both wet and semi-dry electrophoretic film transfer in Western blotting experiments. This product is prepared by conventional method, is premixed powder, does not contain SDS and methanol, can be prepared into 10× electrophoresis transfer buffer, easy to store, easy to operate.

The components of 10× electrophoretic transfer buffer were 250mM Tris and 1.92M Glycine.

Usage:

1. Each bag of powder can be prepared into 1000ml 10×Western Transfer Buffer.

2. Deionized water or double steaming water is used as solvent when preparing.

3. Take out all the powder in the packaging bag and dissolve it in 800ml deionized water or distilled water, and the volume is fixed to 1000ml.

4, this product does not contain SDS, protein molecular weight is large or strong hydrophobicity, in order to increase the film transfer effect, can be appropriate to add a small amount of SDS (reference working concentration of 0.025-0.1%).

5. 1× electrophoresis transfer buffer configuration method: Take 100mL of 10× electrophoresis transfer buffer, fill it with deionized water or distilled water to 800ml, add 200 mL anhydrous methanol before electrophoresis, and mix it well. It is recommended to add methanol on the spot.

Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.

Remark：These protocols are for reference only. Solarbio does not independently validate these methods.

Note：

1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.

2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.

3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.