TBST buffer is an isotonic buffer salt solution commonly used in biology, which is mainly used to wash away non-specific binding antibodies and other reagents on the membrane in Western Blot experiments. Tris-HCl formed a stable PH buffer system, NaCl provided isotonic conditions, and Tween-20 as a detergent could increase the elution capacity of the buffer.

1×TBST buffer composition: 10mM Tris-HCl, 150mM sodium chloride, Tween-20, 0.05% (V/V).

Usage:

1. Each bag of powder and 0.5ml Tween-20 can be mixed to prepare 1000ml of 1×TBST buffer.

2. Deionized water or double steaming water is used as solvent when preparing.

3. Take out all the powder in the packaging bag and dissolve it in 800ml deionized water or distilled water, and the volume is fixed to 1000ml.

4. Add 0.5ml Tween-20 before use and mix well.

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