English
中文
CAS:56092-82-1
Storage:Powder:-20℃，2 years;Insolvent(Mother Liquid):-20℃，6 months;-80℃，1 year
Purity:≥98%
Appearance:White to off-white Solid
Manual Download
Examples of using this product（for reference only）
In Vitro:
Cell（the peripheral blood lymphocytes, 1 μg/ml ionomycin calcium salt, 5h）:
Blood from the M6-immunized (three doses) mice or rhesus monkeys was collected at and 28 days post-infection. Red blood cells were removed, and the peripheral blood lymphocytes were washed and suspended in RPMI-1640 medium supplemented with 10% heat-inactivated fetal bovine serum, 100 U/mL penicillin and 100 μg/mL streptomycin. Then, the cells were cultured for 5 h in the presence of phorbol 12-myristate 13-acetate (PMA) (50 ng/ml;Solarbio),ionomycin calcium salt (1 μg/ml; Solarbio) and GolgiStop (0.67 μl/ml)
Reference：
Xu X, Feng X, Wang L, Yi T, Zheng L, Jiang G, Fan S, Liao Y, Feng M, Zhang Y, Li D, Li Q. A HSV1 mutant leads to an attenuated phenotype and induces immunity with a protective effect. PLoS Pathog. 2020 Aug 10;16(8):e1008703. doi: 10.1371/journal.ppat.1008703. PMID: 32776994; PMCID: PMC7440667.
