| English Name |
Anti-CARM1 Polyclonal Antibody |
| Chinese Name |
蛋白精氨酸N甲基4抗体 |
| Host |
Rabbit |
| Clone Type |
Polyclonal Antibody |
| Subtype |
IgG |
| Dilution Ratio |
WB 1:1000-3000. IHC 1:50-200. |
| Target |
CARM1 |
| Synonyms |
CARM1_HUMAN, Coactivator associated arginine methyltransferase 1, Protein arginine N-methyltransferase 4, PRMT4, PRMT 4, Coactivator-associated arginine methyltransferase 1, Histone arginine methyltransferase CARM 1, Histone-arginine methyltransferase CARM1, Protein arginine methyltransferase, Protein arginine N methyltransferase 4 |
| Background |
Methylates (mono- and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in several proteins involved in DNA packaging, transcription regulation, pre-mRNA splicing, and mRNA stability. Recruited to promoters upon gene activation together with histone acetyltransferases from EP300/P300 and p160 families, methylates histone H3 at 'Arg-17' (H3R17me), forming mainly asymmetric dimethylarginine (H3R17me2a), leading to activate transcription via chromatin remodeling. During nuclear hormone receptor activation and TCF7L2/TCF4 activation, acts synergically with EP300/P300 and either one of the p160 histone acetyltransferases NCOA1/SRC1, NCOA2/GRIP1 and NCOA3/ACTR or CTNNB1/beta-catenin to activate transcription. During myogenic transcriptional activation, acts together with NCOA3/ACTR as a coactivator for MEF2C. During monocyte inflammatory stimulation, acts together with EP300/P300 as a coactivator for NF-kappa-B. Acts as coactivator for PPARG, promotes adipocyte differentiation and the accumulation of brown fat tissue. Plays a role in the regulation of pre-mRNA alternative splicing by methylation of splicing factors. Also seems to be involved in p53/TP53 transcriptional activation. Methylates EP300/P300, both at 'Arg-2142', which may loosen its interaction with NCOA2/GRIP1, and at 'Arg-580' and 'Arg-604' in the KIX domain, which impairs its interaction with CREB and inhibits CREB-dependent transcriptional activation. Also methylates arginine residues in RNA-binding proteins PABPC1, ELAVL1 and ELAV4, which may affect their mRNA-stabilizing properties and the half-life of their target mRNAs. |
| Swiss Prot |
Q86X55 |
| Gene ID |
10498 |
| Subcellular Location |
Cytoplasm Nucleus |
| Immunogen |
A synthetic peptide of human CARM1 |
| Public Immunogen Range |
470-520/608aa |
| Calculated MW |
42/43/63kDa |
| Purification Method |
Octanoic acid-ammonium sulfate precipitation |
| Storage Buffer |
PBS with 50% Glycerol，0.03% Proclin300，0.5% BSA，pH 7.3. |
| Storage |
Store at -20℃. Avoid freeze/thaw cycles. |
| Piece |
Piece |
| Specification |
50ul 100ul |
| Reactivity |
Human Rat Mouse |
| Application |
WB IHC |
English
中文
Manual Download
