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Application：ELISA
Reactivity：Mouse
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：39pg/mL
Storage：2-8℃，6 months
Gene ID：12475
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| Name | Mouse CD14 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | CD14 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Mouse |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 78.125-5000pg/mL |
| Sensitivity | 39pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Swiss Prot | P10810 |
| Gene ID | 12475 |
| Background | CD14 is an acute phase glycoprotein that binds lipopolysaccharide (LPS) endotoxins with cells，thereby signaling the presence of gram-negative bacteria (1-4). Its 11 leucine-rich repeats mediate the interaction with LPS. The 55 kDa form，mCD14，is anchored to the membrane via glycosylphosphatidylinositol (GPI) linkage (1，2，5-7). Human mCD14 shares 63-73% amino acid sequence identity with mouse，rat or rabbit CD14. Soluble forms of CD14，or sCD14，may be secreted prior to GPI linkage or shed by proteolysis or cleavage of the GPI linkage. This variation，plus variable glycosylation，creates forms that may range from 43 to 53 kDa (8-10). In humans，the N terminal 13 kDa，termed presepsin or sCD14-ST，is a small soluble subtype that is found in plasma during sepsis or local infection (11-13). Membrane CD14 is expressed primarily on the cells that are most sensitive to LPS，including monocytes，macrophages and neutrophils (1-3). Lower amounts are detected on other cells，such as B cells，epithelial cells，endothelial cells，and fibroblasts (1，14-16). Soluble CD14 is found in serum，urine and other body fluids (5). |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
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