Rat ACE2 ELISA Kit
Cat.No:SEKR-0085 Solarbio
Reactivity:Rat
Sample Type:Serum,Plasma,Cell Culture Supernatant
Sensitivity:125 pg/ml
Storage:2-8℃,6 months
Gene ID:302668
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My CartReactivity:Rat
Sample Type:Serum,Plasma,Cell Culture Supernatant
Sensitivity:125 pg/ml
Storage:2-8℃,6 months
Gene ID:302668
Qty:
Size:
Name | Rat ACE2 ELISA Kit |
Storage | 2-8℃,6 months |
Detection Target | ACE2 |
Sample Type | Serum,Plasma,Cell Culture Supernatant |
Reactivity | Rat |
Reaction Type | Sandwich-ELISA |
Quantitative/Qualitative | Quantitative |
Detection Range | 250-16000 pg/ml |
Sensitivity | 125 pg/ml |
Specificity | Do not react with Rat ACE2 analogue etc. |
Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
Recovery | 80%-120% |
Assay Time | 3h |
Swiss Prot | Q5EGZ1 |
Gene ID | 302668 |
Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗大鼠ACE2单克隆抗体包被在酶标板上;分别加入梯度稀释的标准品和预稀释的样本,标准品和样本中的大鼠ACE2会与酶标板上的包被抗体充分结合;洗板后加入HRP酶标记的抗大鼠ACE2抗体,该抗体会与板子上包被抗体捕获的标准品和样本中的大鼠ACE2发生特异性结合;洗板后加入显色剂底物TMB,若反应孔中样品存在不同浓度的大鼠ACE2,则HRP会使无色TMB变成不同深浅(正相关)的蓝色物质,加入终止液后反应孔会变成黄色;最后,在λmax=450 nm(OD=450 nm)处测定反应孔样品吸光度(OD),样本中的大鼠ACE2浓度与OD成正比,通过绘制标准曲线和四参数拟合软件便可计算出样本中大鼠ACE2的浓度。 |
Background | Angiotensin-converting enzyme 2 (ACE2),a first homolog of ACE,regulates the renin angiotensin system (RAS) by counterbalancing ACE activity. Accumulating evidence in recent years has demonstrated a physiological and pathological role of ACE2 in the cardiovascular,renal and respiratory systems. ACE2 also has an important role in blood pressure control. This enzyme,an homolog of ACE,hydrolyzes angiotensin (Ang) I to produce Ang-(1-9),which is subsequently converted into Ang-(1-7) by a neutral endopeptidase and ACE. ACE2 releases Ang-(1-7) more efficiently than its catalysis of Ang-(1-9) by cleavage of Pro(7)-Phe(8) bound in Ang II. Thus,the major biologically active product of ACE2 is Ang-(1-7),which is considered to be a beneficial peptide of the RAS cascade in the cardiovascular system. A physiological role for ACE2 has been implicated in hypertension,cardiac function,heart function and diabetes,and as a receptor of the severe acute respiratory syndrome coronavirus. In the acute respiratory distress syndrome (ARDS),ACE,AngII,and AT1R promote the disease pathogenesis,whereas ACE2 and the AT2R protect from ARDS. Importantly,ACE2 has been identified as a key SARS-coronavirus receptor and plays a protective role in severe acute respiratory syndrome (SARS) pathogenesis. Furthermore,the recent explosion of research into the ACE2 homolog,collectrin,has revealed a new physiological function of ACE2 as an amino acid transporter,which explains the pathogenic role of gene mutations in Hartnup disorder. This review summarizes and discusses the recently unveiled roles for ACE2 in disease pathogenesis. |
Unit | Kit |
Specification | 48T 96T |
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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