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Application：ELISA
Reactivity：Human
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：80 pg/ml
Storage：2-8℃，6 months
Gene ID：2324
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| Name | Human VEGF R3/FLT-4 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | VEGF R3/Flt-4 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Human |
| Application | ELISA |
| Reaction Type | Sandwich-ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 156.25-10000 pg/ml |
| Sensitivity | 80 pg/ml |
| Specificity | Do not react with Human VEGF， Human VEGF-C， Human VEGF-D， Human VEGFR1/FcChimera， Human VEGFR2/FcChimera， Mouse VEGFR3/FcChimera etc. |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h |
| Swiss Prot | P35916 |
| Gene ID | 2324 |
| Detection Principle | Solarbio ELISA试剂盒采用基于双抗体夹心法的酶联免疫吸附检测技术。将抗人VEGF R3/Flt-4单克隆抗体包被在酶标板上；分别加入梯度稀释的标准品和预稀释的样本，标准品和样本中的人VEGF R3/Flt-4会与酶标板上的包被抗体充分结合；洗板后加入HRP酶标记的抗人VEGF R3/Flt-4抗体，该抗体会与板子上包被抗体捕获的标准品和样本中的人VEGF R3/Flt-4发生特异性结合；洗板后加入显色剂底物TMB，若反应孔中样品存在不同浓度的人VEGF R3/Flt-4，则HRP会使无色TMB变成不同深浅（正相关）的蓝色物质，加入终止液后反应孔会变成黄色；最后，在λmax=450 nm（OD=450 nm）处测定反应孔样品吸光度（OD），样本中的人VEGF R3/Flt-4浓度与OD成正比，通过绘制标准曲线和四参数拟合软件便可计算出样本中人VEGF R3/Flt-4的浓度。 |
| Background | VEGF R1 (Flt-1)，VEGF R2 (KDR/Flk-1)，and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis，angiogenesis，and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth，while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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