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Application：ELISA
Reactivity：Porcine
Sample Type：Serum，Plasma，Cell Culture Supernatant
Sensitivity：3pg/mL
Storage：2-8℃，6 months
Qty：
Size：
| Name | Porcine IL-3 ELISA Kit |
| Storage | 2-8℃，6 months |
| Detection Target | IL-3 |
| Sample Type | Serum，Plasma，Cell Culture Supernatant |
| Reactivity | Porcine |
| Application | ELISA |
| Quantitative/Qualitative | Quantitative |
| Detection Range | 15.625-1000pg/mL |
| Sensitivity | 3pg/mL |
| Precision | Both intra-plate and inter-plate variation coefficients were less than 10% |
| Recovery | 80%-120% |
| Assay Time | 3h15min |
| Background | Interleukin 3 (IL-3) is a protein that is encoded by the IL3 gene and it is called colony-stimulating factor; mast cell growth factor，MULTI-CSF，MCGF; MGC79398，MGC79399. The protein contains 152 amino acids and its molecular weight is 17 kDa and the naturally occurring IL-3 is glycosylated. IL-3 is produced as a monomer by activated T cells，monocytes/macrophages and stroma cells. The major function of IL-3 cytokine is to regulate blood-cell production. It induces proliferation and differentiation of early pluripotent stem cells and committed progenitors. Interleukin 3 acts by binding to the interleukin-3 receptor. IL-3/Receptor complex induces JAK2/STAT5 cell signalisation pathway. It can stimulate transcription factor c-myc (activation of gene expression) and Ras pathway (suppression of apoptosis). Interleukin 3 has been shown to interact with IL3RA.Interleukin 3 stimulates the differentiation of multipotent hematopoietic stem cells into myeloid progenitor cells or，with the addition of IL-7，into lymphoid progenitor cells. In addition，IL-3 stimulates proliferation of all cells in the myeloid lineage (granulocytes，monocytes，and dendritic cells)，in conjunction with other cytokines，e.g.，Erythropoietin (EPO)，Granulocyte macrophage colony-stimulating factor (GM-CSF)，and IL-6. It is secreted by basophils and activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. |
| Unit | Kit |
| Specification | 48T 96T |
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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