Sodium Citrate buffer(0.01mol/L, pH6.0, powder)
Cat.No：C1010 Solarbio
Appearance：White powder
Storage：Store at RT，1 year.
Purity：0.01M
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> Biochemical Reagents > Universal Buffer > Sodium Citrate buffer(0.01mol/L, pH6.0, powder)
Product Information
| Name | Sodium Citrate buffer(0.01mol/L, pH6.0, powder) |
| Storage | Store at RT，1 year. |
| Appearance | White powder |
| Purity | 0.01M |
| Unit | Bag |
| Specification | 10*1L 10*2L 100*1L 100*2L |
After the cell or tissue is fixed with paraformaldehyde, formaldehyde or other aldehydes, the intracellular antigen will form aldehyde bonds and carboxymethyl bonds, and part of the antigenic determinant will be blocked. At the same time, the cross-linking between proteins will make the antigenic determinant hidden, resulting in weakened staining signal during immune staining, and even some false negative staining results. Therefore, when immunohistochemical staining is performed, antigen repair or exposure is required first, that is, the cross-linking formed between molecules during fixation is destroyed, and the original spatial form of the antigen is restored. So the detection rate of antigen can be improved, the background staining can be reduced, and the accuracy of detection can be improved.
Sodium citrate buffer 0.01mol/L, pH6.0 is a commonly used antigenic repair solution, which can be used for the antigenic repair of paraffin, frozen section and other samples after fixing with paraformaldehyde, formaldehyde or other aldehyde reagents. It can effectively remove the cross-linking between proteins caused by aldehydes fixing agents, and fully expose the epitopes in paraffin sections and other samples, thus greatly improving the effect of immunostaining.
Usually paraffin sections need to be treated with antigen repair, while frozen sections can not be treated with antigen repair. Antigen repair can improve the immunostaining effect of paraffin section and the staining effect of frozen section. When the immunostaining effect of frozen section is not satisfactory, antigen repair should be considered.
According to the calculation of 10ml antigen repair solution required for each film, 1L antigen repair solution can be used for antigen repair of 100 samples.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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