QuickCut restriction endonuclease BsaI
Cat.No:Q2020 Solarbio
Storage:Store at -20℃,1 year.
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Endonuclease >
QuickCut restriction endonuclease BsaIStorage:Store at -20℃,1 year.
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Name | QuickCut restriction endonuclease BsaI |
Storage | Store at -20℃,1 year. |
Unit | Piece |
Specification | 50Rxns |
Fast endonuclides are a series of restriction endonuclides that have been genetically engineered to precisely cut DNA in 5 to 15 minutes, and are suitable for rapid enzyme cutting of plasmid DNA, PCR products or genomic DNA. Fast endonuclease has the following characteristics: it can be completed in 5~15 minutes; Sharing one digestion Buffer greatly simplifies the digestion reaction system. Good redundancy of enzyme activity, easy to cope with substrate excess or difficult template enzyme cutting. In addition, dephosphorylation and ligation reagents are 100% active in the digestion Buffer, supporting a tubeization reaction and improving the enzyme-modified-ligation experience.
REF: MD035
5 '... G G T C T C (N) 1... 3 '
3 '... C C A G A G (N) 5/... The 5' fast enzyme can complete the reaction in 5-15 minutes
Reaction conditions: 10× FastCut Buffer&10× FastCut Color Buffer, 37℃.
Thermal inactivation: 80℃for 20 minutes
Methylation sensitivity: Sensitivity to dcm and mammalian genomic DNA CpG methylation
Asterisk activity: 1 hour incubation does not show asterisk activity, 16 hours may show asterisk activity
Note:Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark:These protocols are for reference only. Solarbio does not independently validate these methods.
Note:
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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Production of Sitobion avenae-resistant Triticum aestivum cvs using laccase as RNAi target and its systemic movement in wheat post dsRNA spray
Click to check >>Author:Asma Rafique, Amber Afroz, Nadia Zeeshan, Umer Rashid, Muhammad Azmat Ullah Khan, Muhammad Irfan, Waheed Chatha, Muhammad Ramzan Khan, Nazia Rehman
IF:3.7520
Publish_to:PLoS One
PMID:37163535