SDS-PAGE loading buffer,5×(with Reductant)
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Cat.No：P1043 Solarbio
Storage：Store at -20℃，1 year
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> Protein and Immunity > Protein Electrophoresis > Protein Electrophoresis Reagents > SDS-PAGE loading buffer,5×(with Reductant)
Product Information
| Storage | Store at -20℃，1 year |
| Unit | Bottle |
| Specification | 10ml |
This product is a modified protein loading buffer that is 5 times less concentrated and safer. Its main components are SDS, reducing agent, bromophenol blue, buffer salt solution and so on. SDS can bind with protein to produce a large amount of negative charge on the protein-SDS complex, then the charge of the protein itself is completely covered by SDS, eliminating the difference in the charge of various proteins. SDS can also break intramolecular and intermolecular hydrogen bonds and destroy the secondary and tertiary structures of protein molecules. Reducing agents can break disulfide bonds between cysteine residues, destroying protein structures and eliminating differences between protein structures. Ultimately no charge and structural differences in the protein (subunit), electrophoresis speed is only related to the size of its molecular weight. Bromophenol blue is used as an indicator during electrophoresis, indicating roughly when the electrophoresis will end.
Note：Product information may be optimized and upgraded. Please refer to the actual label information for accuracy.
Remark：These protocols are for reference only. Solarbio does not independently validate these methods.
Note：
1. The products are all for scientific research use only. Do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Do not store them in ordinary residential areas.
2. For your safety and health, please wear laboratory clothes, disposable gloves and masks.
3. The experimental results may be affected by many factors, after-sale service is limited to the product itself and does not involve other compensation.
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