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Determination of Significance：
As an important class of compounds, amino acids account for 15%-60% of total nitrogen in soil, which are ‘sink’ and ‘source’ in soil nitrogen cycle and plant nutrient supply. Amino acids could be absorbed directly or by mineralization to satisfy the need of plant nitrogen nutrition. Amino acids are also important nitrogen sources for soil microbes and have direct effects on soil microbial community structure, quantity and activity. Study on variation of amino acid contents in soil is of great significance to soil nitrogen cycle and plant ecophysiology.
Measurement Principle：
α-Amino of amino acid could react with hydrated ninhydrin to produce blue purple compound, which has absorption peak at 570 nm, and content of amino acid is calculated by measuring absorbance of 570 nm.
Self Provided：
Spectrophotometer/microplate reader, refrigerated centrifuge, transferpettor, water bath/dry bath, micro glass cuvette/96 well flat-bottom plate, mortar, 30-50 mesh sieve, ice and distilled water.
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